International Journal of Nutrition, Pharmacology, Neurological Diseases

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 4  |  Issue : 4  |  Page : 203--213

Ferulic acid prevents ultraviolet-B radiation induced oxidative DNA damage in human dermal fibroblasts


Kanagalakshmi Ambothi, Rajendra Prasad Nagarajan 
 Department of Biochemistry and Biotechnology, Annamalai University, Chidambaram, Tamil Nadu, India

Correspondence Address:
Rajendra Prasad Nagarajan
Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar 608 002, Chidambaram, Tamil Nadu
India

Aim: Protective effects of ferulic acid (FA) against ultraviolet-B (290-320 nm) radiation induced cellular changes were investigated in human dermal fibroblasts (HDFa). Materials and Methods: HDFa cells pretreated with increasing concentrations of FA (0, 10, 20, 40 μg/ml) for 30 min, were UVB irradiated and different cellular and oxidative end points were analyzed. Results: The percentage of cytotoxicity, intracellular reactive oxygen species (ROS) levels, mitochondrial membrane potential, thiobarbituric acid reactive substances (TBARS) and DNA damage, were significantly increased in 19.8 mJ/cm 2 ultraviolet-B (UVB)-exposed HDFa. Further, exposure to UVB causes significantly decreased antioxidants status in HDFa cells. Treatment of HDFa cells with FA before 30 min of UVB-irradiation significantly restored mitochondrial membrane potential, ROS levels and antioxidant status in HDFa. Further, FA treatment reverted UVB-induced mutagenesis in Ames tester strains and DNA damage in HDFa. Moreover, we noticed increased expression of GADD 45α, XRCC1 and HOGG1 in UVB exposed HDFa. Conversely, FA pretreatment significantly attenuated UVB-induced expression of DNA repair genes in HDFa. Conclusion: The present findings indicate that FA act as a sunscreen rather than working at molecular level to offer photoprotection.


How to cite this article:
Ambothi K, Nagarajan RP. Ferulic acid prevents ultraviolet-B radiation induced oxidative DNA damage in human dermal fibroblasts.Int J Nutr Pharmacol Neurol Dis 2014;4:203-213


How to cite this URL:
Ambothi K, Nagarajan RP. Ferulic acid prevents ultraviolet-B radiation induced oxidative DNA damage in human dermal fibroblasts. Int J Nutr Pharmacol Neurol Dis [serial online] 2014 [cited 2020 Dec 4 ];4:203-213
Available from: https://www.ijnpnd.com/article.asp?issn=2231-0738;year=2014;volume=4;issue=4;spage=203;epage=213;aulast=Ambothi;type=0