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Year : 2011  |  Volume : 1  |  Issue : 1  |  Page : 64-68

Modulations in the haemolymph of silkworm [Bombyx mori (l). (Lepidoptera: Bombycidae)] fed with mulberry leaves augmented with cowpeas (Vigna unguiculata)

1 Department of Zoology, Faculty of Science, Annamalai University, Annamalai nagar-608 002, Tamil Nadu, India
2 Division of Biochemistry, Rani Meyyammai College of Nursing, Faculty of Medicine, Annamalai University, Annamalai nagar-608 002, Tamil Nadu, India

Correspondence Address:
Saravanan Nadanam
Division of Biochemistry, Rani Meyyammai College of Nursing, Faculty of Medicine, Annamalai University, Annamalai nagar - 608 002, Tamil Nadu
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2231-0738.77534

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Aim: To evaluate the effect of mulberry leaves supplemented with cowpeas (Vigna unguiculata) on the haemolymph of silkworm. Materials and Method: Finely powdered V. unguiculata was dissolved in distilled water and diluted to 2.5%, 5%, 7.5%, and 10% concentrations. Fresh mulberry leaves (Morus alba L.) were sprayed with each concentration and fed to silkworms, from third to fifth instar, with five feedings per day. Group 1 larvae received mulberry leaves sprayed with distilled water and served as control, group 2 larvae received 2.5% V. unguiculata-sprayed mulberry leaves, group 3 larvae received 5% V. unguiculata-sprayed mulberry leaves, group 4 larvae received 7.5% V. unguiculata-sprayed mulberry leaves, and group 5 larvae received 10% V. unguiculata-sprayed mulberry leaves. Result: Silkworm larvae fed on M. alba L. (mulberry) leaves enriched with 7.5% concentrations of V. unguiculata, significantly gained more cocoon weight, shell weight and shell/cocoon ratio as compared to those fed on normal MR 2 mulberry leaves. Hence, 7.5% dose of V. unguiculata was fixed as an effective dose. Further, same study was conducted to find out the biochemical changes in the haemolymph occurred on the first day of fifth instar larvae. There was significant increase in the haemolymph glucose, cholesterol, urea, total protein, aspartate transaminase, alanine transaminase, and alkaline phosphatase. But haemolymph uric acid decreased significantly. Conclusion: The results suggest that co-administration of V. unguiculata with mulberry leaves at a concentration of 7.5% enhanced the biochemical reaction involved in the silk production in the silkworm.

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