Figure 6 (a) Effect of ARB on apoptosis. (i) Untreated normal H9c2 cells, white arrows indicate green fluorescence. (ii) ISO (25 μM) red arrows indicate necrotic cells. (iii) ARB (40 μM) blue arrows indicate apoptotic cells. (iv) ARB 40 μM + ISO 25 μM. (b) Percentage of apoptotic cells were calculated by scoring apoptotic and viable cells. The values are given as mean ± SD of six experiments in each group, ANOVA followed by DMRT. *Significant compared to control (P < 0.05). **Significant compared to ISO (P < 0.05). ANOVA, analysis of variance; ARB, arbutin; DMRT, Duncan’s Multiple range test; ISO, isoproterenol hydrochloride; SD, standard deviation.