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  Indian J Med Microbiol
 

Figure 1: Mechanism of actin nucleation by Spir proteins: (a) The tandem WH2 domains are designated as A, B, C, and D, and the corresponding linker regions as L-1, L-2, and L-3. First two actin monomers get bound to WH2-D and WH2-C to initiate the formation of an actin dimer and are stabilized by L-3. Subsequently, G-actin is added to WH2-B and WH2-A to form trimer and tetramer. (b) Four actin monomers are bound to the cluster of four WH2 domains. (c) Further polymerization occurs at the barbed (+) end, whereas Spir proteins remain bound to the pointed end of the actin filament, (Adapted from Kerkhoff, 2006)

Figure 1: Mechanism of actin nucleation by Spir proteins: (a) The tandem WH2 domains are designated as A, B, C, and D, and the corresponding linker regions as L-1, L-2, and L-3. First two actin monomers get bound to WH2-D and WH2-C to initiate the formation of an actin dimer and are stabilized by L-3. Subsequently, G-actin is added to WH2-B and WH2-A to form trimer and tetramer. (b) Four actin monomers are bound to the cluster of four WH2 domains. (c) Further polymerization occurs at the barbed (+) end, whereas Spir proteins remain bound to the pointed end of the actin filament, (Adapted from Kerkhoff, 2006)