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  Indian J Med Microbiol
 

Figure 5: Effect of SM on UVB-induced activation of p53, Bax and caspase-3 and suppression of Bcl-2 in HDFa cells. HDFa cells were exposed to UVB (19.8 mJ/cm2) with or without pretreatment with SM (8, 40 and 80 μM) for 30 min. Cells were harvested at 4 h after UVB exposure, and cell lysates were prepared to determine the activation of p53, Bax and caspase-3 and suppression of Bcl-2 using Western blot analysis. The UVB plus SM treatment at concentrations of 8, 40 and 80 µM significantly down-regulated the p53, Bax and caspase-3 and up-regulated Bcl-2 in a dose-dependent manner (compared with 0 mg/ml control group, P<0.05). The graph represents the quantification results normalized to β-actin levels. Data represent the means ± SD of three individual experiments. *Significantly different from control cells (P<0.05). **Significantly different from UVB-exposed cells (P<0.05)

Figure 5: Effect of SM on UVB-induced activation of p53, Bax and caspase-3 and suppression of Bcl-2 in HDFa cells. HDFa cells were exposed to UVB (19.8 mJ/cm2) with or without pretreatment with SM (8, 40 and 80 μM) for 30 min. Cells were harvested at 4 h after UVB exposure, and cell lysates were prepared to determine the activation of p53, Bax and caspase-3 and suppression of Bcl-2 using Western blot analysis. The UVB plus SM treatment at concentrations of 8, 40 and 80 µM significantly down-regulated the p53, Bax and caspase-3 and up-regulated Bcl-2 in a dose-dependent manner (compared with 0 mg/ml control group, <i>P</i><0.05). The graph represents the quantification results normalized to β-actin levels. Data represent the means ± SD of three individual experiments. *Significantly different from control cells (<i>P</i><0.05). **Significantly different from UVB-exposed cells (<i>P</i><0.05)