International Journal of Nutrition, Pharmacology, Neurological Diseases

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 9  |  Issue : 3  |  Page : 97--104

Preventive Effect of Arbutin on Isoproterenol-Induced Oxidative Stress, Mitochondrial Damage and Apoptosis in H9c2 Cells


Subramanian Sivasangari, Loordhurani Asaikumar, Lakshmanan Vennila, Natesan Vijayakumar 
 Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, India

Correspondence Address:

Aim: This study aims to investigate the potential mechanisms associated with cardioprotective effect of arbutin (ARB) on isoproterenol hydrochloride (ISO) induced cardiotoxicity in H9c2 cardiomyoblast cell lines. Materials and Methods: The effect of the drug on cell morphology was studied by using phase contrast microscope, cell viability was studied by using 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) staining, and reactive oxygen species (ROS) production was estimated by 20-7’dichlorofluorescein diacetate staining. H9c2 cells were treated with ISO to cause cell injury and the effect of the ARB on cell morphology, mitochondrial membrane potential, intracellular ROS generation, cell viability, and apoptosis were studied. Results and Conclusion: The results of this study showed that preadministration of ARB significantly prevented the ISO-induced toxic effects on cell morphology and enhanced the number of viable cells in dose-dependent manner. This study also demonstrates that ROS generation was significantly increased in ISO-administered cells and ISO-induced ROS production was found to be significantly reduced in preadministration of ARB on H9c2 cells. ISO-induced changes in mitochondrial membrane potential of H9c2 cells were remarkably improved with ARB pretreatment. These results clearly suggest that pretreatment of ARB protects the cells against ISO-induced injury through resuming mitochondrial function and regulating apoptosis.


How to cite this article:
Sivasangari S, Asaikumar L, Vennila L, Vijayakumar N. Preventive Effect of Arbutin on Isoproterenol-Induced Oxidative Stress, Mitochondrial Damage and Apoptosis in H9c2 Cells.Int J Nutr Pharmacol Neurol Dis 2019;9:97-104


How to cite this URL:
Sivasangari S, Asaikumar L, Vennila L, Vijayakumar N. Preventive Effect of Arbutin on Isoproterenol-Induced Oxidative Stress, Mitochondrial Damage and Apoptosis in H9c2 Cells. Int J Nutr Pharmacol Neurol Dis [serial online] 2019 [cited 2019 Nov 18 ];9:97-104
Available from: http://www.ijnpnd.com/article.asp?issn=2231-0738;year=2019;volume=9;issue=3;spage=97;epage=104;aulast=Sivasangari;type=0