International Journal of Nutrition, Pharmacology, Neurological Diseases

ORIGINAL ARTICLE
Year
: 2012  |  Volume : 2  |  Issue : 1  |  Page : 31--39

Sesamol modulates ultraviolet-B-induced apoptotic and inflammatory signaling in human skin dermal fibroblasts


Samivel Ramachandran, Nagarajan Rajendra Prasad 
 Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, India

Correspondence Address:
Nagarajan Rajendra Prasad
Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar 608 002, Tamilnadu
India

Aim: Sesamol (SM), a dietary phenolic phytochemical, has been shown to reduce ultraviolet-B (UVB) mediated oxidative damage. The aim of the present study was to investigate the protective mechanism of SM against UVB-induced photoaging, inflammatory and apoptotic signaling in human skin dermal fibroblasts, adult (HDFa) in vitro. Materials and Methods: In this study, we examined the effect of SM on UVB radiation-induced loss of mitochondrial membrane potential (ΔΨm), DNA fragmentation, cell cycle modulation, inflammatory markers [tumor necrosis factor (TNF)-α and nuclear factor (NF)-κB] expression, pro-apoptotic (p53, Bax and caspase-3), and anti-apoptotic marker (Bcl-2) expression in HDFa. We also investigated the effect of SM and/or UVB radiation on matrix metalloproteinase (MMP-2 and MMP-9) activation by gelatin zymograpy in HDFa. Results and Conclusion: SM pretreatment prevented UVB-induced ΔΨm alteration, DNA fragmentation and down-regulated the expressions of apoptotic (p53, Bax and caspase-3) and inflammatory markers (TNF-α and NF-κB) in HDFa. SM also prevented the activation of MMP-2 and MMP-9 in a concentration-dependent manner. Our data indicated the ability of SM to block UVB-induced inflammatory and apoptotic signaling in HDFa. However, further detailed mechanistic approach warrants before claiming this compound for photoprotection.


How to cite this article:
Ramachandran S, Prasad NR. Sesamol modulates ultraviolet-B-induced apoptotic and inflammatory signaling in human skin dermal fibroblasts.Int J Nutr Pharmacol Neurol Dis 2012;2:31-39


How to cite this URL:
Ramachandran S, Prasad NR. Sesamol modulates ultraviolet-B-induced apoptotic and inflammatory signaling in human skin dermal fibroblasts. Int J Nutr Pharmacol Neurol Dis [serial online] 2012 [cited 2020 Feb 29 ];2:31-39
Available from: http://www.ijnpnd.com/article.asp?issn=2231-0738;year=2012;volume=2;issue=1;spage=31;epage=39;aulast=Ramachandran;type=0