Users Online: 954

Home Print this page Email this page Small font sizeDefault font sizeIncrease font size

Home | About us | Editorial board | Search | Ahead of print | Current issue | Archives | Submit article | Instructions | Subscribe | Contacts | Login 
     
ORIGINAL ARTICLE
Year : 2019  |  Volume : 9  |  Issue : 3  |  Page : 97-104

Preventive Effect of Arbutin on Isoproterenol-Induced Oxidative Stress, Mitochondrial Damage and Apoptosis in H9c2 Cells


Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, India

Correspondence Address:
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijnpnd.ijnpnd_24_19

Rights and Permissions

Aim: This study aims to investigate the potential mechanisms associated with cardioprotective effect of arbutin (ARB) on isoproterenol hydrochloride (ISO) induced cardiotoxicity in H9c2 cardiomyoblast cell lines. Materials and Methods: The effect of the drug on cell morphology was studied by using phase contrast microscope, cell viability was studied by using 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) staining, and reactive oxygen species (ROS) production was estimated by 20-7’dichlorofluorescein diacetate staining. H9c2 cells were treated with ISO to cause cell injury and the effect of the ARB on cell morphology, mitochondrial membrane potential, intracellular ROS generation, cell viability, and apoptosis were studied. Results and Conclusion: The results of this study showed that preadministration of ARB significantly prevented the ISO-induced toxic effects on cell morphology and enhanced the number of viable cells in dose-dependent manner. This study also demonstrates that ROS generation was significantly increased in ISO-administered cells and ISO-induced ROS production was found to be significantly reduced in preadministration of ARB on H9c2 cells. ISO-induced changes in mitochondrial membrane potential of H9c2 cells were remarkably improved with ARB pretreatment. These results clearly suggest that pretreatment of ARB protects the cells against ISO-induced injury through resuming mitochondrial function and regulating apoptosis.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed149    
    Printed14    
    Emailed0    
    PDF Downloaded22    
    Comments [Add]    

Recommend this journal