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ORIGINAL ARTICLE
Year : 2015  |  Volume : 5  |  Issue : 2  |  Page : 82-88

Mutagenic evaluation and spectroscopic characterization of flavonoidal fraction of Apium leptophyllum (Pers.) fruit


1 Department of Pharmaceutical Sciences, NIMS Institute of Pharmacy, Jaipur, Rajasthan, India
2 Department of Pharma Analysis and Quality Assurance, Institute of Pharmacy and Technology, Cuttack, Odisha, India
3 Department of Pharmacognosy and Phytochemistry, Vedica College of Pharmacy, Bhopal, Madhya Pradesh, India
4 Department of Pharmacology, NIMS Medical College, NIMS University, Jaipur, Rajasthan, India

Correspondence Address:
Himanshu Bhusan Sahoo
NIMS Institute of Pharmacy, NIMS University, Jaipur - 303 121, Rajasthan
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2231-0738.153798

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Objective: The present study was aimed to evaluate the antimutagenic potential of flavonoidal fraction of Apium leptophyllum fruits (FFALF) and isolate the bioactive phytoconstituents from that fraction along with their subsequent characterization. Materials and Methods: For mutagenicity study, a single application of FFALF at doses of 5 mg/kg, 10 mg/kg, and 20 mg/kg of body weight were orally administered to Swiss mice, 24 h prior the i.p. administration of cyclophosphamide (50 mg/kg). After 24 h of administration of cyclophosphamide, the rodents were sacrificed and the aberrant chromosomal cells as well as micronuclei formations from the bone marrow cells were compared with the positive control. Then, the phytoconstituents were isolated from FFALF using column chromatography and characterized by spectroscopic evidences of Fourier transform infrared spectroscopy (FTIR), mass spectroscopy (MS), hydrogen-1 nuclear magnetic resonance ( 1 H NMR), and carbon-13 nuclear magnetic resonance ( 13 C NMR). Results: The pretreatment of FFALF significantly (P < 0.01) reduced the frequency of micronuclei formation and the incidence of chromosomal aberration in a dose-dependent manner from the bone marrow cells of mice as compared to the cyclophosphamide induced group. The cumulative spectral data showed the presence of two flavonoids isolated from FFALF, i.e., apigenin and quercetin. Conclusion: In the present study, apigenin and quercetin have been successfully isolated from FFALF. The isolation of the above characterized flavonoids exerts antimutagenic activity against cyclophosphamide-induced genetic abnormalities in Swiss mice. In the future, it may be useful to prepare plant-based pharmaceutical preparation to treat various genetic or immunological disorders.


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