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ORIGINAL ARTICLE
Year : 2014  |  Volume : 4  |  Issue : 3  |  Page : 146-152

Alteration in antioxidants level and lipid peroxidation of patients with neurodegenerative diseases {Alzheimer's disease and Parkinson disease}


1 Department of Chemical Pathology, College of Health Sciences, Ladoke Akintola University of Technology, Osogbo, Osun, Nigeria
2 Department of Medicine, Neurology Unit, College of Health Sciences, Ladoke Akintola University of Technology, Osogbo, Osun, Nigeria
3 Department of Clinical Pathology, Lagos University Teaching Hospital, Idi Araba, Nigeria
4 Department of Clinical Pathology, Neuropsychiatric Hospital, Yaba, Lagos, Nigeria

Date of Submission16-Jan-2014
Date of Acceptance25-Feb-2014
Date of Web Publication16-May-2014

Correspondence Address:
Ogunro Paul Sunday
Department of Chemical Pathology, College of Health Science, Ladoke Akintola University of Technology, Osogbo
Nigeria
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2231-0738.132671

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   Abstract 

Objective: To assess the level of oxidative stress (OS) and the antioxidants that play a prominent role in OS of neurodegenerative diseases; also to test the hypothesis that OS is associated with neuronal degeneration in patients with neurodegenerative diseases {Parkinson's Disease (PD) and Alzheimer's Disease (AD)}. Materials and Methods: A total of 28 AD, 42 PD patients and 42 healthy controls aged 60-80 yrs were recruited for the study. Plasma total antioxidant status (TAS), erythrocyte malondialdehyde (MDA) and glutathione (GSH) concentrations were determined. Erythrocyte antioxidant enzyme activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and glucose-6-phosphate dehydrogenase (G6PD) were measured by using standard methods. Results: Plasma TAS was significantly reduced (P < 0.05) in AD and PD subjects when compared with the controls. Erythrocyte antioxizdant enzymes activities of SOD, GSH-Px, CAT and activity of G6PD were significantly reduced (P < 0.01) in AD and PD when compared with that of controls. However, erythrocyte level of MDA in AD and PD subjects were significantly increased (P < 0.01) compared to the controls. Erythrocyte GSH level was significantly reduced (P < 0.01) in AD subjects and (P < 0.05) in PD subjects when compared with the control. Strong significant (P < 0.01) correlation was obtained between the marker of OS (MDA) and SOD among PD and AD patients. Conclusion: The present study reveals elevated OS and strong correlation between SOD and MDA. This indicates that reduced SOD plays a prominent role in the increase of OS in neuronal degeneration.

Keywords: Antioxidants, lipid peroxidation, neurodegerative diseases


How to cite this article:
Sunday OP, Adekunle MF, Temitope OT, Richard AA, Samuel AA, Olufunminyi AJ, Elizabeth OO. Alteration in antioxidants level and lipid peroxidation of patients with neurodegenerative diseases {Alzheimer's disease and Parkinson disease}. Int J Nutr Pharmacol Neurol Dis 2014;4:146-52

How to cite this URL:
Sunday OP, Adekunle MF, Temitope OT, Richard AA, Samuel AA, Olufunminyi AJ, Elizabeth OO. Alteration in antioxidants level and lipid peroxidation of patients with neurodegenerative diseases {Alzheimer's disease and Parkinson disease}. Int J Nutr Pharmacol Neurol Dis [serial online] 2014 [cited 2019 May 26];4:146-52. Available from: http://www.ijnpnd.com/text.asp?2014/4/3/146/132671


   Introduction Top


Free radicals are molecules with unpaired electron in their outer orbit. Free radicals have very important role in origin of life and biological evolution, having beneficial. effects on the organisms. They pose a serious threat to vital organs, tissues, polyunsaturated fatty acids (PUFAs) of cell membranes and nucleic acids of cells. [1] The activities of free radicals in many age-related diseases have long been of interest, and there is substantial evidence linking cancer, diabetes mellitus, atherosclerosis, neurodegenerative diseases (Alzheimer's and Parkinson's disease), rheumatoid arthritis, ischemic/reperfusion injury, obstructive sleep apnea, cardiovascular disease, hypertension and ageing to free radical-induced alterations. [2],[3]

Alzheimer's Disease (AD) is a progressive neurodegenerative disorder characterized by cognitive and memory decline, speech loss and personality changes resulting in severe dementia; it is one of the major cause of admissions to nursing homes. [4] AD affects 6-8% of the population aged 65 years and 30% of the population aged 85 years. [5] To date, despite intensive efforts, the pathogenesis of AD remains elusive, and this incomplete understanding of disease pathogenesis has greatly affected the development of accurate animal and cellular models, and thereby retarded the development of therapeutic modalities. Several independent hypotheses have been proposed to link the pathologic lesions and neuronal cytopathology with, among others, apolipoprotein E genotype, [6] hyperphosphorylation of cytoskeletal proteins and amyloid-β metabolism. [7],[8] However, none of these hypotheses alone is sufficient to explain the diversity of biochemical and pathologic abnormalities of AD, which involve a multitude of cellular and biochemical changes. [9],[10],[11] The biochemical mechanism of the pathogenesis of this disease is still unknown, but there is accumulating evidence that suggests a key role of oxidative stress in the pathophysiology of AD. Free radicals produced during oxidative stress are speculated to be pathologically important in AD and other neurodegenerative diseases. [12] Studies have shown that the increase in the level of oxidized protein in Alzheimer's disease is associated with loss of the activity of the 20S proteasome, which represents a major enzyme for the degradation of oxidized proteins. [13],[14] The current research in AD is mainly focused in the post-mortem characterization of the biochemical and pathological alterations present in the brain. [15] Studies in plasma and cerebrospinal fluid of AD patients for the evaluation of the levels of specific vitamins or antioxidants and biomarkers of lipid peroxidation are scanty.

Parkinson's disease (PD) is the second most common neurodegenerative disorder after AD, affecting approximately 1 per cent of the population older than 50 years. [16] Parkinson's disease is a chronic neurodegenerative disease characterized by progressive loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc). The development of PD in patients becomes clinically apparent with severe motor symptoms, including uncontrolled resting tremor, bradykinesia, rigidity, and postural imbalance. [17] In most cases, these symptoms appear after 70-80% of SNpc dopaminergic neurons are lost. [18] The exact etiology of PD remains unclear, but what is clearly known is that the disease is of multi-factorial in nature, including both environmental and genetic factors, which result in development of oxidative stress in specific areas of the brain. [19] Some hypotheses try to explain the nature and causes of this type of cell degeneration. Several studies show that oxidative stress (OS) and mitochondrial dysfunction are two cardinal factors, leading to the development of this process. [20],[21] Current concepts of the pathogenesis of PD center on the formation of reactive oxygen species and the onset of oxidative stress leading to oxidative damage to SNpc.

There are reports suggesting a decrease in antioxidant capacity and other antioxidant enzyme activities with increase in various markers of lipid peroxidation in neurodegenerative diseases. [22],[23] Although the changes in lipid peroxidation and antioxidant defenses are documented in the SNpc of PD patients, the studies of the central nervous system (CNS) are beset with the complexity of direct investigation because of the inaccessibility of the neural tissue, and hence the difficulty in obtaining a brain biopsy, until after the death of the afflicted individual. [23]

In healthy tissues, one of the main sources of free radicals is the mitochondria. This is because these organelles are responsible for more than 90% of cellular oxygen consumption and the radicals in biological systems always, ultimately are generated by the metabolism of oxygen by this route. However, over the course of evolution, cells have constructed enzymatic machinery to combat oxidative stress. For example, superoxide dismutase (SOD) is an enzyme that catalyzes the reaction of the superoxide radical (O 2· - ) to hydrogen peroxide and molecular oxygen (O 2 .) is found in the matrix of mitochondria, which is the site of oxidative phosphorylation. [24] Catalase (CAT) and glutathione peroxidase (GSH-Px) are two other antioxidant enzymes that catalyze the reaction of hydrogen peroxide to molecular oxygen and water, thereby reducing a major source of oxidative stress. All of these enzymes have been shown to confer protection to the neurons against oxidative stress. It is therefore imperative to develop suitable peripheral markers, which can help in the diagnosis and monitoring of neurodegenerative diseases during life. Hence, the aim of the study is to assess the antioxidants capacity and the antioxidants that play a prominent role in development of oxidation stress in the blood of neurodegenerative diseases (AD and PD) patients; and also the level of correlation between antioxidants and marker of oxidative stress.


   Materials and methods Top


Subjects

The protocol for the study was approved by the Ethical Committee of the Ladoke Akintola University of Technology, College of Health Sciences, Osogbo and Lagos University Teaching Hospital, Lagos, Nigeria. A total of 28 AD patients and 42 PD patients aged 60-80 yrs were recruited from the Neurology Unit of the Department of Internal Medicine of the teaching hospitals. Diagnosis of AD was made using the National Institute for Neurological Disorders/Alzheimer's Disease and Related Disorders (NINDS/ADRDA) [25] criteria. Whereas, the diagnostic criteria for PD were based on the UK PD society brain bank [26] and the selection of PD subjects were based on the following inclusion criteria: tremor, muscular rigidity, brady kinesia, loss of postural reflexes and the phenomenon of freezing foot. The exclusion criteria include vertical gaze impairment, marked autonomic disturbance, bladder and bowel involvements, secondary  Parkinsonism More Details, associated dementia, apraxia or pseudobulbar palsy and patients on antipsychotic drugs. The 42 healthy control subjects belong to the same social, age/sex and ethnic background who were recruited from the general population. These control subjects were recruited based on their medical history and physical examination. Their cognitive function was assessed using mini-mental state examination (MMSE) [27] and they did not show signs of dementia.

Analytical procedure

After explaining the rationality of the study, written/informed consent was obtained from the subjects/careers prior to their inclusion in the study. About 10 ml of venous blood was collected after an overnight fast from the venous vein at the antecubital fossa without stasis. Blood samples were collected by venipuncture into vacutainer tubes containing lithium heparin and were centrifuged at 2000 g for 10 min. The plasma was carefully removed and the erythrocyte pellet was washed twice with equal volumes of saline and centrifuged at 2000 g for 15 min. The washed red blood cells were then hemolyzed in distilled water (1:4, v/v) and by freezing and thawing. The hemolyzate was centrifuged and the supernatant and plasma were then stored at -20°C until they were analyzed.

Glucose-6-phosphate dehydrogenase (G6PD) activities were assayed according to the procedure described by Beutler. [28] Malondialdehyde (MDA) level was determined using the method of Draper and Hadley [29] based on the reaction of MDA with thiobarbituric acid (TBA) at 95°C. In the TBA test reaction, MDA and TBA react to form a pink pigment with an absorption maximum at 532 nm. The reaction was performed at pH 2-3 at 95°C for 15 min. The sample was mixed with 2.5 volumes of 10% (w/v) trichloroacetic acid to precipitate the protein. The precipitate was pelleted by centrifugation and an aliquot of supernatant was reacted with 0.67% TBA in a boiling water-bath for 15 min. After cooling, the absorbance was read at 532 nm. Arbitrary values obtained were compared with a series of standard solutions (1, 1, 3, 3 tetramethoxypropane).

Measurement of total antioxidant status (TAS) in the plasma was performed by using a method contained in the commercial kit from Randox Laboratories (Randox Laboratories Ltd, Diamond Road, Crumlin, Co. Antrim, Ireland). [30] The assay was calibrated using 6-hydroxy-2, 5, 8-tetra-methylchroman-2-carboxylic acid (trolox). The results were expressed as mmol/L of trolox equivalent. Measurement of erythrocyte GSH-Px (EC # 1.11.1.9) activity was performed using a commercial kit RANSEL from Randox Laboratories (Randox Laboratories Ltd, Diamond Road, Crumlin, Co. Antrim, Ireland). GSH-Px catalyses the oxidation of glutathione (GSH) to glutathione disulphide (GSSG) by cumenehydroperoxide, in the presence of glutathione reductase and nicotinamide adenine dinucleotide phosphate (NADPH), GSSG is immediately converted to GSH with a concomitant oxidation of NADPH to Nicotinamide adenine dinucleotide phosphate (NADP + ) according to the method of Paglia and Valentine. [31] SOD (EC. 1.15.1.1) activity was assayed by employing xanthine/xanthine oxidase assay commercial kit RANSOD from Randox Laboratories (Randox Laboratories, Crumlin, Antrim, UK). [32] The results of SOD activity were normalized to the hemoglobin content in the erythrocyte lysate and expressed as U/gHb. GSH concentration in erythrocytes was determined using Glutathione Assay Kit; GSH reductase converts oxidized GSH in the sample to reduce GSH. Reaction with reduced GSH, Ellman's Reagent [5, 5'-dithiobis (2-nitrobenzoic acid)] releases a colored product, monitored at 415 nm using a microplate reader. The intensity of the color produced is proportional to GSH concentration, and the calculations were made according to the model curve for GSH, and expressed in μmoles GSH/g Hb. [33] The CAT (EC 1.11.1.6) peroxidative activity was measured by the reaction of formaldehyde produced from methanol with Purpald to produce a chromophore according to the method of Johansson and Hakan Borg; [34] activity was quantified by measuring the absorbance at 540 nm and comparing the results with those obtained with formaldehyde calibrators.

Statistical analysis

Statistical analysis was done with the SPSS version 11 software. All data were expressed as mean ± SD. Statistical significance was analyzed with the paired Student's t-test. Correlations were performed by Pearson's method. P value of less than 0.05 was considered statistically significant.


   Results Top


The anthropometric measurements and physical clinical parameters of AD and PD subjects and the controls are presented in [Table 1]. Both the subjects and the control are normotensive. The mean ± SD of biochemical parameters are shown in [Table 2]; plasma concentration of TAS of 1.03 ± 0.08 mmol/L trolox equivalent for AD subjects and 1.16 ± 0.12 mmol/L trolox equivalent for PD subjects were significantly reduced (P < 0.05) compared to the control. However, the erythrocytes MDA of 4.63 ± 0.95 nmol/ml for AD subjects and 5.63 ± 0.74 nmol/ml for PD subjects were significantly increased (P < 0.01) compared to the control. The mean activity of G6PD of 8.79 ± 1.51 U/gHb for AD subjects and 9.04 ± 1.05 U/gHb for PD subjects were significantly reduced (P < 0.01) compared to the control. The erythrocytes activities of antioxidant enzymes GSH-Px, SOD and CAT were 39.71 ± 3.82 U/gHb, 675.16 ± 87.07 U/gHb and 859.05 ± 87.74 U/gHb respectively for AD subjects and 37.80 ± 5.37 U/gHb, 704.82 ± 98.19 U/gHb and 909.27 ± 89.31 U/gHb respectively for PD subjects and were significantly reduced (P < 0.01) compared to the control. Likewise, the erythrocytes concentration of GSH (3.37 ± 0.62 μmol/g Hb) for the AD subjects was significantly reduced (P < 0.01) compared to the control and 4.09 ± 0.97 μmol/g Hb for PD subjects was also significantly reduced (P < 0.05) compared to the control.
Table 1: Clinical parameters of neurodegenerative diseases and controls


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Table 2: The (mean±SD) plasma concentrations of measured biochemical parameters of the subjects and the controls


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A negative correction was obtained between GSH-Px and MDA (r = -0.3642, P = 0.019) for AD subjects and (r = -0.3927, P = 0.0154) for PD subjects. Likewise, a strong negative correlation was obtained between SOD and MDA (r = -0.4759, P = 0.0092) for AD subjects and (r = -0.4855, P = 0.0086) for PD subjects. Similarly, a negative correlation was obtained between CAT and MDA (r = -0.2851, P = 0.0436) for AD subjects and (r = -0.3758, P = 0.0186) for the PD subjects. A negative correlation was also obtained between CAT and MDA (r = -0.3057, P = 0.0239) for AD subjects and (r = -0.3253, P = 0.0209) for the PD subjects. [Figure 1] shows the comparison between the measured biochemical parameters of the subjects and the controls in graphical form [Table 3].
Table 3: Correlations between MDA and antioxidants


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Figure 1: Levels of biochemical parameters for the subjects and the controls

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   Discussion Top


Oxidative stress, characterized by an imbalance between exposure to free radicals or other reactive species and antioxidant defenses, has been implicated in the pathogenesis of neurodegenerative diseases such as AD and PD [2],[3] and it may be related to changes in mitochondrial function and protein clearance. [20] In this study, the systemic decrease in multiple antioxidants (such as plasma TAS, erythrocytes GSH, G6PD and erythrocyte antioxidant enzymes activities) with increase in marker of oxidative stress (MDA) provides evidence that peripheral indices of oxidative damage are elevated in AD/PD patients compared to controls.

G6PD is a key enzyme involved in the synthesis of NADPH, which is essential for keeping the normal level of reduced GSH; G6PD deficient erythrocytes are particularly sensitive to oxidative stress and reduction in G6PD activity may result in reduction of GSH level. [35] Both G6PD activity and GSH concentration are significantly reduced in AD and PD subjects compared to the controls in our study. This finding was in agreement with the finding of Sian et al., [36] on post-mortems studies, they reported that GSH levels in the SNpc of PD patients are remarkably lower than those of healthy subjects (60% compared to control subjects) while oxidized GSH (GSSG) levels are slightly increased. Although GSH is not the only antioxidant molecule reported to be altered in PD, it is hypothesized that the magnitude of its depletion is the earliest indicator of nigrostriatal degeneration. [37]

Gu et al., [38] on their study reported that GSH levels are depressed in AD cingulated cortex and AD substantia innominata, while Liu et al., [39] found these reduced levels only in red blood cells of male AD patients. However, increased GSH levels have been observed by Adams et al., [40] in the midbrain and in the caudate nucleus, while normal GSH contents was reported by Perry et al., [41] The dissenting results are most likely due to differences in techniques or difficulty in sample collection after death of AD patients. However, it has been observed that GSH protects cultured neurons against oxidative damage resulting from β-peptide and 4-hydroxynonenal (HNE), a lipid peroxidation product that is increased in AD. [42] This decrease in GSH may be due to its increased utilization to maintain a reduced cellular environment, which has been disturbed due to oxidative stress. Since GSH is a naturally occurring antioxidant, therefore it seems that much of it is being consumed in protecting the cell from cellular damage caused by cytotoxic radicals.

The SOD, CAT and GSH-Px are three primary enzymes, involved in direct elimination of active oxygen species (hydroxyl radical, superoxide radical, hydrogen peroxide) whereas GSH reductase, G6PD, and cytosolic GST are secondary enzymes, which help in the detoxification of reactive oxygen species (ROS) by decreasing peroxide levels or maintaining a steady supply of metabolic intermediates like GSH and NADPH necessary for optimum functioning of the primary antioxidant enzymes. [43] In the present study, a significant reduction in the activities of SOD, CAT, GSH-Px and G6PD with increase in MDA level of the erythrocytes for AD and PD patients was in line with an earlier report by other groups. [44],[45] The increase in the oxidative stress due to low activity of antioxidant enzymes might pave way for many secondary complications and may contribute to the neurodegeneration in AD and PD. SOD showed a significant reduction in activity in neurodegenerative disease patients, which may lead to an increase of superoxide radical. Zhang et al., [46] reported that some of the deleterious effects of N-methyl-4-phenyl-1, 2, 3, 6- tetrahydropyridine (MPTP) on striatal dopaminergic nerve terminals are mediated by both superoxide and hydroperoxides and they occur prior to dopaminergic neurodegeneration in the SN. Superoxide radicals can also react with nitric oxide to generate peroxynitrite (ONOO−), a putative neurotoxin. [47] A significant reduction in both CAT and GSH-Px activities in effect can increase the production of highly deleterious H 2 O 2 . Since SOD, CAT, GSH-Px and GSH are significantly reduced in AD and PD subjects in this study, TAS concentration follows suit. We also recorded a significant negative correlation between the SOD and the marker of oxidative stress (MDA); SOD is an enzyme that catalyzes the reaction of the superoxide radical (O 2−) to hydrogen peroxide and molecular oxygen (O 2 ). This antioxidant enzyme is found in the matrix of mitochondria which is the site of oxidative phosphorylation in neuronal cell. [24] However, no significant correlation was obtained between TAS and MDA. This can be attributed to the fact that other antioxidants such as the transition metal and the small molecule "sacrificial" antioxidants not assayed in this study may compensate for the reduction in other antioxidants in which all contribute to the systemic TAS.


   Conclusion Top


Our study shows elevated oxidative stress and reduced activities of erythrocyte antioxidant enzymes such as SOD, GSH-Px and CAT. This revealed the possible role of free radicals in neurodegenerative diseases. Strong significant correlation between SOD and MDA shows that SOD reduction as result of increased free radical production contribute more to oxidative stress in mitochondria than other antioxidants, which may be responsible for the loss of cholinergic, noradrenergic and dopaminergic neurons that play a vital role in the pathogenesis of neurodegenerative diseases. Antioxidant therapy of SOD composition will be of immense benefit in neuronal mitochondria protection.

 
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    Figures

  [Figure 1]
 
 
    Tables

  [Table 1], [Table 2], [Table 3]


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