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ORIGINAL ARTICLE
Year : 2014  |  Volume : 4  |  Issue : 2  |  Page : 95-103

Pharmacological evaluation of calendula officinalis L. on bronchial asthma in various experimental animals


1 Department of Pharmaceutical Sciences, Dr. H.S. Gour Vishwavidyalaya, Sagar, Madhya Pradesh, India
2 Department of Pharmacology and Experimental Biology, Vedica College of Pharmacy, Ram Krishna Dharmarth Foundation University, Bhopal, Madhya Pradesh, India
3 Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, India
4 School of Pharmaceutical Education and Research, Berhampur University, Berhampur, Odisha, India
5 School of Pharmaceutical Science, Siksha O Anusandhan University, Bhubaneswar, Odisha, India
6 Department of Pharmaceutical Chemistry, Jayamukhi Institute of Pharmaceutical Science, Warangal, Andhra Pradesh, India

Correspondence Address:
Himanshu Bhusan Sahoo
Department of Pharmacology and Experimental Biology, Vedica College of Pharmacy, Ram Krishna Dharmarth Foundation University, Bhopal 462 033, Madhyapradesh
India
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Source of Support: Vedica College of Pharmacy, RKDF University, Bhopal - 462 033, Madhya Pradesh, India,, Conflict of Interest: None


DOI: 10.4103/2231-0738.129595

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Background: Calendula officinalis Linn. (Asteraceae) is an aromatic herb growing in the forests of India, China, Central Europe, and some tropical areas. The study was designed to investigate the scientific basis for the traditional claim of C. Officinalis on asthma. In the present study, methanol extract of whole plants was evaluated for preliminary phytochemical screening and antiasthmatic activity on different animals. Subjects and Methods: The asthmatic activity was evaluated by histamine- or acetylcholine-induced bronchospasm in guinea pigs, compound 48/80-induced mast cell degranulation in wistar rats, histamine-induced constriction on isolated guinea pig trachea, and ovalbumin-induced sensitization in mice at different dose levels of C. Officinalis. The preconvulsion dyspnoea time at 0 th and 7 th days at the dose of 250 and 500 mg/kg in guinea pig, the percentage of granulated and degranulated mast cell of at the dose of 600, 800, 1000 μg/mL in rats, muscular contraction at the dose of 600, 800, and 1000 μg/mL on isolated guinea pig trachea and the inflammatory cell count, that is, eosinophils, neutrophills, lymphocytes, macrophages, interleukin (IL)-4, IL-5, and immunoglobulin-E (IgE) from bronchoalveolar lavage fluid at the dose levels of 50, 100, and 250 mg/kg in mice were evaluated and compared with respective control groups. Results: Phytochemical studies revealed the presence of flavonoids, steroids, saponin, terpenoids, lignins, and phenolic compounds in the extract. In addition, the treatment of methanolic extract of C. officinalis (MECO) significantly (P < 0.001) decreased the bronchospasm induced by histamine or acetylcholine in guinea pigs, degranulation mast cell in rats, histamine-induced constriction on isolated guinea pig trachea, and the level of inflammatory cells as compared with inducer groups. The antiasthmatic activity was potentiated in all the doses in dose-dependent manner. Conclusion: The present study concludes that the antiasthmatic activity may be due to the presence of above phytoconstituents by antihistaminic, anticholinergic, antispasmodic, and mast cell stabilizing property.


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