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ORIGINAL ARTICLE
Year : 2013  |  Volume : 3  |  Issue : 1  |  Page : 39-45

Anticoagulant and antioxidant activity of sulfated chitosan from the shell of donacid clam Donax scortum (Linnaeus, 1758)


1 Center for Advanced Studies in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, India
2 Department of Pharmacology, Chettinad Hospital and Research Institute, Kelambakkam, India
3 Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India

Correspondence Address:
Annaian Shanmugam
CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai - 608 502
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2231-0738.106990

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Objectives: Sulfated chitosan was prepared from the shell of donacid clam Donax scortum by following demineralization, deproteinization, deacetylation and sulfating with chlorosulfonic acid. Results: FT-IR spectrum proved the structure of sulfated chitosan. The yield, sulfate content and molecular weight was found 80.30%, 14.2% and 18.65 × 10 4 Da. Their anticoagulant activity was determined for human plasma with respect to the activated partial thromboplastin time (APTT) and prothrombin time (PT) and it was 6.45 and 1.73 IU, respectively. The antioxidant activity of sulfated chitosan was evaluated as radical scavengers against superoxide anion (71.46% at 0.5 mg/ ml) and hydroxyl radicals (64.73% at 3.2 mg/ml). The antioxidant activity of sulfated chitosan was evaluated as radical scavengers against superoxide anion and hydroxyl radicals of 71.46% at 0.5 mg/ml and 64.73% at 3.2 mg/ml. The chelating ability and reducing power of sulfated chitosan was 43.24 at 10 mg/ml and 0.578 at 0.75 mg/ml. IC 50 values of scavenging abilities on superoxide anion and hydroxyl radicals were 0.398 and 0.527 mg/ml whereas those of chelating ability and reducing power were 5.01 and 0.728 mg/ml. Conclusion: These results indicated that sulfated chitosan from D. scortum had potent anticoagulant and antioxidant activities.


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