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ORIGINAL ARTICLE
Year : 2013  |  Volume : 3  |  Issue : 1  |  Page : 29-33

Isolation and characterization of phosphate-solubilizing bacterial species from different crop fields of Salem, Tamil Nadu, India


Department of Biotechnology, Faculty of Engineering, Vinayaka Missions Engineering College, Vinayaka Missions University, Ariyanoor, Tamil Nadu, India

Correspondence Address:
Muruhan Sridevi
Department of Biotechnology, Faculty of Engineering, Vinayaka Missions Engineering College, Vinayaka Missions University, Salem, Tamil Nadu
India
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Source of Support: None, Conflict of Interest: None


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Phosphate-solubilizing bacterial strains (PSB) were isolated from maize, onion, jasmine, and tomato rhizosphere soils from four different localities of Salem (Tamil Nadu) - (i) Ammapalayam, (ii) Attayampatty, (iii) Seeragapadi, and (iv) Ariyanoor. Twelve efficient PSB isolates were selected from the colonies based on their ability to form clear zone on Pikovskaya's agar medium. The isolated PSB released high amount of phosphorus from calcium phosphate. The efficient bacterial strains isolated from rhizosphere soils released high amount of phosphorus. Oligonucleotide primers in RAPD characterization showed genetic variation among the bacterial strains. Amplified fragments showed 40% shared and 20% unique polymorphic bands. The rest 40% was monomorphic bands.


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