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ORIGINAL ARTICLE
Year : 2012  |  Volume : 2  |  Issue : 3  |  Page : 210-216

Radiosensitizing effect of sesamol on human cervical carcinoma cells


Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar, India

Correspondence Address:
Nagarajan Rajendra Prasad
Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar - 608 002, Tamil Nadu
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2231-0738.99472

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Aim: To determine the radiosensitizing effect of sesamol in cervical carcinoma cell line (HeLa) in vitro. Materials and Methods: Sesamol-pretreated (10 μg/ml) HeLa cells were exposed to 4 Gy γ-radiation and the cellular changes were estimated by lipid peroxidation, changes in the enzymatic and non-enzymatic antioxidant status, oxidative DNA damage, % of growth inhibition, and morphological changes in the irradiated and/or sesamol-treated HeLa cells. Results: Sesamol treatment prior to irradiation significantly increased the levels of thiobarbituric acid reactive substances, (TBARS), lipid hydroperoxides (LPH), and conjugated dienes (CD) in HeLa cells. On the other hand, sesamol treatment before irradiation significantly decreased the levels of reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase activities (GPx). Further, we observed increased % tail DNA, tail length, and tail moment in sesamol plus γ-irradiated cells when compared to irradiation alone. The % of cell death was also increased in sesamol plus γ-irradiated cells, particularly at 48 h incubation. Conclusion: Sesamol pretreatment prior to irradiation enhances the radiation effects in HeLa cell line. This study supports the use of dietary phytochemicals such as sesamol in practical radiotherapy.


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