Users Online: 440

Home Print this page Email this page Small font sizeDefault font sizeIncrease font size

Home | About us | Editorial board | Search | Ahead of print | Current issue | Archives | Submit article | Instructions | Subscribe | Contacts | Login 
     
ORIGINAL ARTICLE
Year : 2012  |  Volume : 2  |  Issue : 1  |  Page : 45-52

Apigenin protects gamma-radiation induced oxidative stress, hematological changes and animal survival in whole body irradiated Swiss albino mice


1 Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, India
2 Division of Radiology, Dr. Kamakshi Memorial Hospital, Chennai, Tamilnadu, India

Correspondence Address:
Nagarajan Rajendra Prasad
Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar 608 002, Tamilnadu
India
Login to access the Email id

Source of Support: Indian council of Medical Research Ref.No: 3/2/2/3/10-NCDIII, IRIS ID: 2009-08970), Conflict of Interest: None


DOI: 10.4103/2231-0738.93134

Rights and Permissions

Aim: The present study was undertaken to find out the possible radioprotective efficacy of apigenin against whole body gamma (γ)-irradiation, oxidative damage and hematological alterations in Swiss albino mice. Materials and Methods: The percentage of mice surviving 30 days, of exposure to radiation dose (7-11 Gy) was used to construct survival dose response curves. Apigenin (15 mg/kg body weight) was administered intraperitonially (i.p) for 7 consecutive days, once daily, and then the mice were exposed to single dose of 7 Gy of γ-radiation. Mice were sacrificed at 24 hours post irradiation, and liver and intestine were taken for various biochemical estimations viz. lipid peroxidation (LPO) markers [thiobarbituric acid reactive substances (TBARS), conjugated dienes (CD) and lipid hydroperoxides (LOOH)], antioxidant status [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione (GSH)]. Further blood was collected for the hematological studies [Haemoglobin (Hb) content, red blood cell (RBC) and white blood cell (WBC) count]. Results: Apigenin 15 mg/kg body weight elevated radiation LD 50 from 8.2 to 10 Gy, indicating the dose modifying factor (DMF) of 1.21. It was found that there was an increased LPO level with decreased antioxidant status in 7 Gy irradiated Swiss albino mice. It has been also observed that Hb content, WBC and RBC count was decreased in irradiated Swiss albino mice. Conversely, significant decrease in LPO and restoration of antioxidant status and hematological changes was observed in apigenin pretreated group. Conclusion: Hence, apigenin pretreatment renders protection against 7 Gy radiation-induced biochemical and hematological alterations in Swiss albino mice.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed6672    
    Printed199    
    Emailed1    
    PDF Downloaded429    
    Comments [Add]    
    Cited by others 13    

Recommend this journal