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ORIGINAL ARTICLE
Year : 2011  |  Volume : 1  |  Issue : 1  |  Page : 69-72

Role of Barley in the improvement of Nephrocalcinosis in experimental rabbit model


1 Department of Pharmacology and Therapeutics, Al-Yarmouk University, Baghdad, Iraq
2 Department of Pharmacology, Al- Diwanyia University, Al- Diwanyia, Iraq

Date of Submission17-Oct-2010
Date of Acceptance01-Jan-2011
Date of Web Publication11-Mar-2011

Correspondence Address:
Rafi A.M. Al-Razzuqi
Department of Pharmacology and Therapeutics, Al-Yarmouk University, Baghdad
Iraq
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2231-0738.77535

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   Abstract 

Introduction: Nephrocalcinosis is a state of deposition of calcium phosphate or oxalate in the renal parenchyma. It may occur in patients with renal tubular acidosis, vitamin D intoxication, and hyperparathyroidism. Aim: According to traditional medicine, which mentioned Barley as a herbal remedy for relieving renal pain, we attempted to evaluate the effectiveness of barley in improving nephrocalcinosis induced by a large dose of oxalic acid in a rabbit model. Materials and Methods: Fourteen healthy rabbits were allocated to two groups. 2 hours before induction of nephrocalcinosis, a group received water and the other received aqueous extract of barley and continued feeding for ten days. Blood samples were collected for biochemical analysis before induction and in the tenth post-induction day. Urine samples were taken to estimate urinary ca+2 levels and crystals. The histopathological examination was carried to check for crystal deposits in renal tissues. Results: The results showed a significant reduction in blood urea nitrogen and serum Na+ levels with insignificant change in serum creatinine and K+ in comparison with the control group. It revealed a significant reduction in calcium deposition from renal parenchyma after 10 days of treatment, with improvement in nephrocalcinosis severity. Conclusion: Barley showed a significant diuretic and attenuating effect on reducing calcium deposits from renal parenchyma.

Keywords: Barley, nephrocalcinosis, oxalic acid, renal tubular acidosis


How to cite this article:
Al-Razzuqi RA, Al-Ebady ZA. Role of Barley in the improvement of Nephrocalcinosis in experimental rabbit model. Int J Nutr Pharmacol Neurol Dis 2011;1:69-72

How to cite this URL:
Al-Razzuqi RA, Al-Ebady ZA. Role of Barley in the improvement of Nephrocalcinosis in experimental rabbit model. Int J Nutr Pharmacol Neurol Dis [serial online] 2011 [cited 2019 Oct 13];1:69-72. Available from: http://www.ijnpnd.com/text.asp?2011/1/1/69/77535


   Introduction Top


Nephrocalcinosis (NC) is a state of deposition of calcium in the form of phosphate or oxalate in the renal parenchyma that can impair kidney function.[1] It can occur in patients with renal tubular acidosis, hyperparathyroidism, vitamin D intoxication, and healing of renal tuberculosis etc. [2] The fragments of calcium salts may break free from the kidney to provide nuclei for the formation of different types and sizes of stones accompanied by many symptoms. [3] They may be consequences of genetic, dietary, water intake, environmental, and occupational factors. [4] In traditional medicine, some medicinal plants like Barley were used to relieve renal pains. [5] This study was made to evaluate the possible nephroprotective effect of aqueous extract of Hordeum distichum (Barley) in experimental model of Nephrocalcinosis (NC).


   Materials and Methods Top


In this study, 14 healthy rabbits weighing 900-1,100 g were used, which was approved by the ethics committee in the Al-Yarmouk University. The animals were supplied by animals' house of college of medicine. Animals were kept in separate cages provided with a wide wire mesh floor. They were fed standard oxoid pellets and water ad libitum. The animals were allocated to two groups (each group contained seven animals).

G1 (control group) received 3 ml of water orally 2 hours before induction of NC by oxalic acid (HandW England) as a single dose of 333 mg/kg orally using a gastric tube.

G2 was given aqueous extract of Barley 1 g/kg orally as a single daily dose started at 9 am, followed by oxalic acid as a single dose of 333 mg/kg orally using a gastric tube at 11 am for induction of NC. The animals were continuously fed the aqueous extract for 10 days post induction.

The aqueous extract of barley is prepared by diluting one volume of well grinded plant to 10 volume of water at 80 °C in stopper flask. After shaking well, it is allowed to stand for 10 minutes then cooled and filtered to be used within 12 hours. [6] The blood samples were collected from marginal ear vein for biochemical analysis and renal functions, before induction of NC to determine the normal values of blood urea nitrogen (BUN), serum creatinine, Na + and K + by using spectrophotometer method [6] on the first and tenth day after induction. Urine samples were taken from the animals by catheterization after anaesthetizing them on the last day of the study to estimate urine Ca +2 levels and search for crystals. Histopathological examination was carried to check for crystal deposits in renal tissues with the help of a polarized microscope after fixation and staining of specimens. [7] The results obtained were analyzed and assessed.


   Statistical Analysis Section Top


We transformed the hazard ratios occurred during the research to their natural logarithms by calculating standard errors and corresponding confidence intervals to account for statistical heterogeneity between our study and Ahmad's study. [9] The data were analyzed using SPSS version (version 12). We carried out group analyses based on the parameters changes before and after the induction of nephrocalcinosis in the study models besides we calculated the standard deviation of each parameter. Significance was set at P<0.05.


   Results Top


The results of this study revealed significant elevation in the levels of BUN, both serum creatinine and K + , with significant reduction of serum Na + levels in the control group as compared to the levels of pre-induction state [Table 1], [Table 2], [Table 3] and [Table 4]. The results of barley (group 2) revealed a significant reduction in BUN (5.18 ± 0.16 vs 7.3 ± 0.2) mmol/L and reduction in serum Na + levels (137.6 ± 0.4 vs 142.16 ± 0.7) mmol/L, with P < 0.05. There is insignificant change in serum creatinine (97.0 ± 0.03 vs 97.3 ± 0.5) mmol/L and serum K + (4.3 ± 0.16 vs 4.2± 0.05) mmol/L in comparison with the control group after one day. The change in these levels became more evident after 10 days.
Table 1: Mean BUN, S creatinine, K+, Na+, and urine Ca+2 levels of the studied animals measured before induction of NC

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Table 2: Mean BUN and S creatinine levels of the studied groups measured after induction of NC

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Table 3: Mean S K+ and S Na+ levels of the studied groups measured after induction of NC

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Table: 4 Mean urine Ca+2 levels of the studied groups measured 10 days after induction of NC

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   Discussion Top


Nephrocalcinosis was induced in the experimental model by using a single large dose (333 mg/kg) of oxalic acid (a highly oxidising and strong Ca +2 chelator) and this dose had been selected after several trials. [8] We did not use Ahmad's method [9] (where, a single oral daily dose of oxalic acid 200 mg/day was given to the animals over a period of two weeks) to induce NC, as it is material and time-consuming method. In our model of NC, significant elevation in BUN and serum creatinine levels observed after induction of NC [Table 2], [Table 3]. The idea to use some herbals like barley is to evaluate its effects in improvement of NC. Barley is a highly alkalising food, [10] contains a variety of antioxidants including 'natural scavenge reactive oxygen' derivative (SOD).[11] Research at the University of California Davis had demonstrated that a flavonoid in barley grass known as 2-0-glycosylisovitexin (2-0-GIV) is a potent antioxidant in protecting against fat oxidation in human cells. [12] Barley grass is high in calcium, iron, all essential amino acids, chlorophyll, flavonoids, vitamin B12, vitamin C, and many minerals, as well as enzymes. It produces significant lowering effect in BUN and serum Na + levels with the increase in urine Ca +2 levels compared to normal values [13] and a reduction of its concentration in renal tissue [Figure 1], [Figure 2] and [Figure 3]. This is due to its diuretic effect, as it reduces the activity of antidiuretic hormone on renal tubules and prevents sodium reuptake, thus increasing the tubular water bulk. [14] Some researchers thought that this effect related to natural complexity of essential amino acids that causes water and electrolytes expulsion. [15]
Figure 1: Normal histological appearance of renal tissue of rabbit (crystal-free) in the control group. ×40.

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Figure 2: Heavy calcium oxalate crystals deposition in renal tissues of rabbits receiving oxalic acid. ×100.

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Figure 3: Mild calcium oxalate crystals deposition in renal tissues of rabbits receiving oxalic acid and Barley. ×100.

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   Conclusions Top


Herbs such as Barley have a significant diuretic and attenuating effect in reducing calcium deposits in renal tissues, with a significant effect on lowering oxalic acid toxicity through its diuretic action.


   Acknowledgement Top


We thank Dr Thulfuqar R Al-Majeed (Department of Medical Laboratory Sciences, Al-Yarmouk University) for helping in examination of the blood samples along with his guidance.

 
   References Top

1.Dorland Medical Dictionary,2002.  Back to cited text no. 1
    
2.Christopher H, Edwin R, Chilver S, John A, Burtis C and Ashood E . Davidson's principles and practice of Medicine. 19th ed. Churchill Livingstone; 2004. p. 594-9.  Back to cited text no. 2
    
3.Drach GW. Urinary lithiasis, Camphil urology. WB Sanders Comp; 1998. p. 2085-100.  Back to cited text no. 3
    
4.Leyva M. The role of dietary calcium in disease prevention. J Okla-State Med Assoc 2003;96:272-5.  Back to cited text no. 4
    
5.David L, Hoffman E. The herbalist and version. Hopki's Technology; 1993.  Back to cited text no. 5
    
6.Burtis CA, Ashood ER. Tietiz text book of clinical chemistry. WB Sanders Comp; Vol 2, 1999. p. 1059-60.  Back to cited text no. 6
    
7.Putt N, Fredrick A. Manual of histopathological staining methods. New York: John Wilay and Sons; 1972. p. 335.  Back to cited text no. 7
    
8.Mendonca DG, Martin LA, Baxmann AC, Nishua IL. Effect of an oxalate load on urinary oxalate excretion in ca+2 stone formation. Nephro Divison universida de federal de Sao Paulo Brazil, 2003;13:39- 46.  Back to cited text no. 8
    
9.Ahmad AF. Evaluation of the effect of succinic acid administration on the urinary excretion of oxalate urolithiasis. Thesis to Mousal University; 2008.  Back to cited text no. 9
    
10.Forber B, Perrson T. kidney stone. The urology group board, 2004;  241-3.  Back to cited text no. 10
    
11.Al-Jawad FH, Hoshi A. Nephroprotective effect of some antioxidants in induced acute renal failure. Iraqi J Med Sci 2011 [In Press].  Back to cited text no. 11
    
12.Tilgnen S. Medical herbalism, clinical and case studies. 1st edition , medical economic company , New jersey, 2001 .  Back to cited text no. 12
    
13.Smith LH. Urolithiasis. In: Schrier RW, Gotts CC, editors. Disease of kidney. WB Sanders Comp.; vol 1, 1996. p. 785-813.  Back to cited text no. 13
    
14.Ives HE. Diuretic agents. Basic and clinical pharmacology edition. In: Katzung BG, Masters SB Treven AJ, editors. 11th international edition. McGraw Hill; 2010.  Back to cited text no. 14
    
15.Finkel R, Cubeddu LX, Clark MA. Pharmacology, 4th international edition. Lippincott Williams and Wilkins; 2009. p. 367-81.  Back to cited text no. 15
    


    Figures

  [Figure 1], [Figure 2], [Figure 3]
 
 
    Tables

  [Table 1], [Table 2], [Table 3], [Table 4]



 

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